Automated And Scalable Closed System Platform For CAR T Cell Isolation And Activation

By Meås, Hilde Almåsbak, Ida C. Schrøder, Katrine Alfsnes, Nicolay R. Nilssen, Sebastian Mevatne, Jenny M. Rasmussen, Kok Shyong Chong, Ming Tiong Sia, Steven Lim, Shan Hua Dong, Woon Liang Terence Soh, Autumn D. Wilbur, Roy A. Thompson, Danny Hernandez, Bernard Boo, Jimmy ZHU, Hilde Nyheim, James Green, Ian Dillon, Eugene L. Kang, Heidi C.Vebø , Tuva Holt Hereng , Øystein Åmellem Cellular Medicine Oslo R&D, Norway, BioProduction, Thermo Fisher Scientific

The Thermo Fisher Scientific Gibco™ CTS™ DynaCellect™ Magnetic Separation System and fit for purpose consumables have been developed for automation of T cell isolation and activation using magnetic beads, such as Gibco™ CTS™ Dynabeads™ CD 3 /CD 28. The automated instrument performs cell separation and bead removal in a closed system by utilizing an integrated magnet rocker and fluidic paths.

The system is highly scalable allowing up to 1 liter of reaction volume for the isolation of up to 10 billion target cells with throughput time of approximately 100 minutes Similarly, bead removal is achieved at a flow rate of 50 mL/min for rapid processing of large volumes