Application Note

An End-To-End, Semi-Continuous Process For mAb Production

Source: Cytiva
Monoclonal Antibody Purification Platform Using High-Capacity Protein A And Mixed-Mode Chromatography

A complete process for production of a biopharmaceutical consists of a great number of distinct unit operations, for example, cell culture, filtration, and chromatography. Linking these different steps together to a fully continuous process can be a challenging task, and studies in the area of continuous processes typically deals with single unit operations, such as perfusion cell culture or continuous chromatography. Examples where two or more unit operations are connected and integrated are therefore of great interest for understanding the technical solutions as well as the approach for overall control.

This application note demonstrates how the different unit operations in a laboratory-scale monoclonal antibody (mAb) process can be connected and integrated into a semicontinuous process. In brief, the mAb process consists of perfusion cell culture, continuous capture chromatography, viral inactivation (VI), post VI filtration, batch polishing chromatography, and a final pH adjustment step.

access the Application Note!

Get unlimited access to:

Trend and Thought Leadership Articles
Case Studies & White Papers
Extensive Product Database
Members-Only Premium Content
Welcome Back! Please Log In to Continue. X

Enter your credentials below to log in. Not yet a member of Bioprocess Online? Subscribe today.

Subscribe to Bioprocess Online X

Please enter your email address and create a password to access the full content, Or log in to your account to continue.

or

Subscribe to Bioprocess Online