Advanced Analytical Profiling Of MSC-EVs Highlights Tissue- And Donor-Dependent Heterogeneity

By Madeline Cramer, PhD, Stephen Lenzini, PhD, Elie Zakhem, PhD, and Jon Rowley, PhD

When developing a scalable MSC-EV manufacturing process, cell source selection is more consequential than it may appear. This poster presents a head-to-head comparison of EVs derived from bone marrow, umbilical cord, and adipose tissue MSCs cultured in RoosterNourish-MSC-XF and RoosterReplenish-MSC-XF across 3D bioreactor and 2D platforms. You will see how particle size, tetraspanin marker abundance, and immunomodulatory potency shift with tissue source, and how donor identity influences yield and CD73 enzymatic activity within a single tissue type.

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