Adjusting Pipetting Variables To Optimize Methods

Simple adjustments to some of the pipetting variables, sometimes referred to as liquid class settings, directly affect volume transfer accuracy. Challenging liquid transfers, such as those involving different liquids with varying viscosity or low surface tension properties, are common in molecular biology assays, and experienced manual pipette operators often adjust their technique without thinking. They do things like slowing down the pipetting speed, pausing more (or less), or adding a blowout. But when an assay is transferred to an automated system, those adjustments need to be explicitly programmed into the liquid handler to ensure accurate and precise liquid handling. Watch this overview to see how you can adjust pipetting variables to help optimize automated liquid handler (ALH) methods.