Adherent PSC Expansion In Nunc Cell Factory Systems: Key Considerations For Scalable Manufacturing

Two strategies dominate PSC expansion at scale: adherent multilayer culture and suspension, and the adherent route offers a scale-out path that keeps media, handling techniques, and growth conditions continuous with flask and plate workflows. This application note covers the practical variables that emerge in Nunc Cell Factory systems: coating volumes by matrix, seeding density adjustments, cell loss at harvest, and microscopy in a 10-layer format. It also presents immunocytochemistry and trilineage differentiation results from cells expanded in a 10-layer system, along with tubing, pump, and automation considerations for closed workflows.

Explore the details to see how these choices fit together in a scalable adherent process.