Application Note

Adenovirus Production In Single-Use ReadyToProcess WAVE™ 25 Bioreactor System

Cells

Upstream virus production for vaccine manufacturing is typically performed in stirred-tank bioreactor systems, but rocking bioreactor systems offer the benefits of low minimum working volumes that can shorten the seed train prior to inoculation, and agitation can be adjusted to a smooth rocking motion for optimized growth of sensitive cells.  This application note describes the production of adenovirus in HEK293 suspension cells in a rocking bioreactor. Triplicate cultures demonstrate a reproducible and robust batch production of adenovirus.

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