A New Specialist For Oligonucleotides With Advantages

Oligonucleotide analysis requires robust methods to handle long polar chains, often leading laboratories to Ion Pairing Reversed Phase (IP-RP) chromatography. However, the aggressive reagents necessary for this technique frequently degrade standard silica gels. In Part 8 of this nine-part Oligos Made Easy series, we examine how hybrid silica technology addresses this durability issue while optimizing ionic and RP interactions.

Beyond durability, comparative data demonstrates that switching columns does not require sacrificing performance for cost; direct method transfers often yield faster retention times with identical resolution. Furthermore, utilizing columns with lower back pressure can extend instrument life and reduce clogging risks, making high-efficiency analysis accessible even without specialized (U)HPLC systems. Access the full article below for data comparing performance against established industry standards and tips for maintaining column longevity.