Application Note

A New, Innovative Process For Clarifying And Sterile Filtering Cells For Protein Purification Workflows

Source: Pall
Sterile pharma manufacturing discovery 450x300

In recent years, biopharmaceutical research processes have demonstrated major improvements in the quality and recovery of mAbs, which to an extent have been associated with culturing the expressing cell lines at high cell densities. This work has generated a great challenge in cell clarification, sterilization, and further downstream processing. These processes must remove large amounts of biomass and increased levels of contaminating cell debris generated during cell culture and harvesting.

This study’s objective was to assess the efficiency of the 24-well, multi-layer clarification and sterile filtration plate and the 24-well sterile filtration plate when used with mammalian cell suspensions. High-density CHO cell suspension, artificially concentrated, and HEK293T cell cultures were used with the 24-well filter plates processed by vacuum or centrifugation.

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