Harvest & Collection

PRODUCTS

  • IADA-2050CH
    Amplifier / Doubler / Amplifier: IADA-2050CH

    The IADA-2050CH is an integrated MMIC active doubler fabricated with GaAs Schottky diodes. This operates over a guaranteed 10 to 25 GHz input frequency range or a doubled output frequency range of 20 to 50 GHz.

  • Culture bioreactor
    Leverage Bioreactor Capacity When You Need It With Culture Bioreactors

    Gain access to Culture bioreactors when you need rapid overflow experimental capacity.

    Benefits

    Progress studies faster

    Run overflow projects and conduct parallel experiments with Culture to reduce time and alleviate bottlenecks.

    Stay agile in your capacity planning

    Scale your workload at Culture according to your current needs. Respond rapidly to surging demands.

    Eliminate your capital equipment spend

    Leverage Culture’s facility quickly to avoid building out lab space, spending capital on equipment, and hiring specialized staff.

  • e-Builder-Screenshot_Water-Online-450x300_42224
    Trimble e-Builder

    As the industry’s most advanced digital construction management system, Trimble e-Builder enables efficiencies across the entire design-build lifecycle, with a smooth transition to the operation phase. Reduce risk, enhance collaboration and improve outcomes using the industry-leading digital project delivery software for owners.

  • CellXpress.ai System
    AI System For Cell Culture Processes

    Automate the entire cell culture process to improve workflows and make assays more reliable and reproducible.

  • tycon
    TY-CON™ Conductive Gasket

    The patented U.S. Pipe TY-CON Conductive Gaskets are for use in Ductile-Iron pipe and fittings provided with the time proven TYTON JOINT®, and HDSS® restrained joint. TY-CON gaskets are intended to provide pipe joint conductivity for thawing service connections in cold climates.

WHITE PAPERS AND CASE STUDIES

NEWS

ABOUT

Harvest and Collection

Harvest and collection is the process used to retrieve biological agents and vaccines present in cell cultures or to harvest and collect stem cells from blood or bone marrow. Many bioresearch companies have sophisticated systems for harvesting and collecting cells. These systems make counting cells easier and they also protect against contamination.

Cells are harvested once the cells reach a density level in the cell culture medium that precludes further growth. The best time to harvest cells is when they are in a confluent state (at least 50% of the culture dish is covered and before 100% of the dish is covered).

Cells can be harvested using on of three methods:  Mechanical, using Proteolytic enzymes, or using EDTA.  Mechanical harvesting uses a rubber spatula to remove the cells from the growth surface or culture. This method is quick but can cause many cells to die because it is highly disruptive. This method is favored when harvesting lots of different samples of cells to prepare extracts.

In this case, viability of the cells doesn’t matter. Three enzymes; Trypsin, Collagenase, and Pronase can be used in combination with EDTA. The combination of these enzymes with the EDTA makes cells detach from the growth medium.

This method is easy but also has a downside. It can damage the cell surface by eating up exposed cell surface proteins. EDTA can be used alone to detach cells from their medium and it is gentler than using trypsin.

Normally, collected cells are placed in a new suspension, or growth medium to continue growing new cultures. This is referred to as passaging or splitting the cells.