Harvest & Collection

PRODUCTS

  • infoworks-ICM
    InfoWorks ICM

    InfoWorks ICM is an advanced, integrated catchment modeling software with cloud capabilities that model complex hydraulic and hydrologic network elements quickly and accurately in a collaborative environment.

  • Research laboratory microscope-GettyImages-1564542843
    Formulation Development Solutions

    Accelerate your drug development with our comprehensive pre-formulation, formulation, and novel delivery system expertise, tailored to optimize physical form, enhance solubility, and support scale-up.

  • Flex-Line Feature
    Robotic Pharmaceutical Aseptic Filling System

    Fast-track your market entry with our robust aseptic filling platform. The Flex-Line™ Robotic Filler provides full integration of Scientific Products filling and Comecer isolation technologies for a seamless process from order through Validation.

  • Esco - DFBG3
    Pharmacon™ Downflow Booth Generation 3

    Esco Pharma's Downflow Booth Generation 3 (DFBG3) is a new generation of Esco powder containment booth which provides simplicity with assured quality.

  • GettyImages-1175279159 powder
    Onsite Preclinical Services To Support Your Product Development: Powder Micromeritics

    Understanding the fine details of powder properties is crucial in the pharmaceutical industry, where the behavior of particulate matter can affect manufacturability and the efficacy of the final product.

WHITE PAPERS AND CASE STUDIES

NEWS

ABOUT

Harvest and Collection

Harvest and collection is the process used to retrieve biological agents and vaccines present in cell cultures or to harvest and collect stem cells from blood or bone marrow. Many bioresearch companies have sophisticated systems for harvesting and collecting cells. These systems make counting cells easier and they also protect against contamination.

Cells are harvested once the cells reach a density level in the cell culture medium that precludes further growth. The best time to harvest cells is when they are in a confluent state (at least 50% of the culture dish is covered and before 100% of the dish is covered).

Cells can be harvested using on of three methods:  Mechanical, using Proteolytic enzymes, or using EDTA.  Mechanical harvesting uses a rubber spatula to remove the cells from the growth surface or culture. This method is quick but can cause many cells to die because it is highly disruptive. This method is favored when harvesting lots of different samples of cells to prepare extracts.

In this case, viability of the cells doesn’t matter. Three enzymes; Trypsin, Collagenase, and Pronase can be used in combination with EDTA. The combination of these enzymes with the EDTA makes cells detach from the growth medium.

This method is easy but also has a downside. It can damage the cell surface by eating up exposed cell surface proteins. EDTA can be used alone to detach cells from their medium and it is gentler than using trypsin.

Normally, collected cells are placed in a new suspension, or growth medium to continue growing new cultures. This is referred to as passaging or splitting the cells.