Harvest & Collection

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    Access Or Service Cleanroom Hatches

    Access or Service hatches range in size, shape and function. Some service hatches are fitted with an enclosure shell for housing devices, with these type of hatches the primary barrier is maintained, regardless if the hatch door is open or closed.

  • VPX3469a
    300 kW Magnetron Transmitter: 3469 Series

    CPI EDB offers the 3469 magnetron transmitter system for use as a high-power source of RF power for outdoor-mounted radar applications. The transmitter cabinet contains the high voltage power supply, solid-state switch, and the high voltage tank assembly which includes the pulse transformer, energy storage high voltage capacitor, and filament power supply.

  • AH - PAM-0101
    10 MHz – 1 GHz RF Preamplifer: PAM-0101

    The PAM-0101 RF preamplifier will provide reliable, repeatable measurements. An ideal solution and a standard workhorse of any EMC test house for compliance testing.

  • PBS Biotech - PBS-1 bioreactor
    Mini Vertical-Wheel Bioreactor: PBS-0.1

    The PBS-0.1 Mini single use bioreactor is compatible with 60mL-100mL working volumes and is the best starting point for optimizing cell therapies by leveraging the benefits of Vertical-Wheel mixing that can be easily scaled up for larger volumes.

  • Sentinel 4000 Drop Through System
    The Sentinel 4000 Drop Through System

    The Thermo Scientific Sentinel 4000 Drop Through System, or Sentinel 4000D, featuring multiscan technology with up to five user-selectable frequencies of detection, provides improved sensitivity and detection of contaminants in drop through applications including granular and powdered food products, cosmetics, and chemicals.

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Harvest and Collection

Harvest and collection is the process used to retrieve biological agents and vaccines present in cell cultures or to harvest and collect stem cells from blood or bone marrow. Many bioresearch companies have sophisticated systems for harvesting and collecting cells. These systems make counting cells easier and they also protect against contamination.

Cells are harvested once the cells reach a density level in the cell culture medium that precludes further growth. The best time to harvest cells is when they are in a confluent state (at least 50% of the culture dish is covered and before 100% of the dish is covered).

Cells can be harvested using on of three methods:  Mechanical, using Proteolytic enzymes, or using EDTA.  Mechanical harvesting uses a rubber spatula to remove the cells from the growth surface or culture. This method is quick but can cause many cells to die because it is highly disruptive. This method is favored when harvesting lots of different samples of cells to prepare extracts.

In this case, viability of the cells doesn’t matter. Three enzymes; Trypsin, Collagenase, and Pronase can be used in combination with EDTA. The combination of these enzymes with the EDTA makes cells detach from the growth medium.

This method is easy but also has a downside. It can damage the cell surface by eating up exposed cell surface proteins. EDTA can be used alone to detach cells from their medium and it is gentler than using trypsin.

Normally, collected cells are placed in a new suspension, or growth medium to continue growing new cultures. This is referred to as passaging or splitting the cells.