Harvest & Collection

PRODUCTS

  • teledyne
    HiRel Semiconductors Releases Wideband 50 GHz RF Switch

    Teledyne HiRel Semiconductors has announced the release of the TDSW050A2T, a high-performance, rad-tolerant RF switch designed for demanding aerospace and defense applications.

  • GettyImages-1339300422 CAR T-Cell Immunotherapy
    Immunotherapy Development

    Immunotherapy is an evolving and promising cancer treatment that works by stimulating the immune system. More biologically relevant human primary cells are now the chosen starting material for immunotherapy development. Historically, scientists routinely used immortalized cell lines in the research and development of therapies as they offer an inexpensive and stable platform. However, they are not fully representative of what is going on in vivo.

  • Gallery-1_Left_TSU-4RW-N6-2
    Undercounter Lab Refrigerator

    PHCbi brand's 3.0 cu.ft. (85 L) undercounter refrigerator is designed to be incorporated into ADA compliant workspaces and fits under most tables, cabinets, and benches. Its convenient glass door provides visibility into the interior of the unit to avoid unnecessary door openings. Temperature performance for your critical storage items in your tight spaces.  Units can be stacked with an adapter to maximize valuable laboratory floor space. This space-saving lab refrigerator includes an automatic defrost function and is ENERGY STAR® certified.

  • GettyImages-1394004470 ovarian cancer
    Oncology Consulting Solutions

    Access specialized data and expertise to help navigate all stages of product development and support your success in oncology.

  • Hull i-Dositecno
    Pharmaceutical Labeling Equipment For Injectable Products

    Ensuring you have equipment that accurately and rapidly assembles syringes and cartridges is crucial to the success of your pharmaceutical manufacturing workflow. The Hull i-Dositecno SY pharmaceutical labeling machine harnesses dual-function robotics for plunger insertion and labeling to ensure the safety and sterility of your product.

    • Versatile – ideal for syringes or cartridges in sterile, clean, ready-to-fill (SCF) tubs
    • Cleanroom ready
    • Fully compliant – 21 CFR Part 11 software and IQ/OQ validation packages available

WHITE PAPERS AND CASE STUDIES

NEWS

ABOUT

Harvest and Collection

Harvest and collection is the process used to retrieve biological agents and vaccines present in cell cultures or to harvest and collect stem cells from blood or bone marrow. Many bioresearch companies have sophisticated systems for harvesting and collecting cells. These systems make counting cells easier and they also protect against contamination.

Cells are harvested once the cells reach a density level in the cell culture medium that precludes further growth. The best time to harvest cells is when they are in a confluent state (at least 50% of the culture dish is covered and before 100% of the dish is covered).

Cells can be harvested using on of three methods:  Mechanical, using Proteolytic enzymes, or using EDTA.  Mechanical harvesting uses a rubber spatula to remove the cells from the growth surface or culture. This method is quick but can cause many cells to die because it is highly disruptive. This method is favored when harvesting lots of different samples of cells to prepare extracts.

In this case, viability of the cells doesn’t matter. Three enzymes; Trypsin, Collagenase, and Pronase can be used in combination with EDTA. The combination of these enzymes with the EDTA makes cells detach from the growth medium.

This method is easy but also has a downside. It can damage the cell surface by eating up exposed cell surface proteins. EDTA can be used alone to detach cells from their medium and it is gentler than using trypsin.

Normally, collected cells are placed in a new suspension, or growth medium to continue growing new cultures. This is referred to as passaging or splitting the cells.