Harvest & Collection

PRODUCTS

  • Gallery-1_Left_TSU-4RW-N6-2
    Undercounter Lab Refrigerator

    PHCbi brand's 3.0 cu.ft. (85 L) undercounter refrigerator is designed to be incorporated into ADA compliant workspaces and fits under most tables, cabinets, and benches. Its convenient glass door provides visibility into the interior of the unit to avoid unnecessary door openings. Temperature performance for your critical storage items in your tight spaces.  Units can be stacked with an adapter to maximize valuable laboratory floor space. This space-saving lab refrigerator includes an automatic defrost function and is ENERGY STAR® certified.

  • Vaccine Vials GettyImages-1316775813
    Automatic High-Speed Visual Inspection Of Pharmaceutical Containers

    CS, MS, VI: Our basic platforms for automatic high-speed inspection of vials, ampules, cartridges, and syringes

    All three standard machines inspect up to 600 containers/minute, in addition to vials, ampules, cartridges, and syringes with liquid contents, as well as vials with lyo product. Depending on the inspection requirements, we can integrate any inspection technology of our Seidenader Solutions and add a machine module for leak testing.

  • Flex-Line Feature
    Robotic Pharmaceutical Aseptic Filling System

    Fast-track your market entry with our robust aseptic filling platform. The Flex-Line™ Robotic Filler provides full integration of Scientific Products filling and Comecer isolation technologies for a seamless process from order through Validation.

  • FMS Automation Module
    Pharmaceutical Automated Sample Handling System For Headspace Testing

    This collaborative automated sample handler eliminates manual sample handling enabling testing of large sample sets and collection of statistically significant data, while freeing analyst’s time. The FMS Automation Module has a sample capacity of hundreds of vials and can complete headspace testing with a throughput as high as 300 samples per hour.

  • HLM-40ABH_450x300
    High Power 40GHz Limiter: HLM-40ABH

    The HLM-40ABH is a high-power, wideband signal limiter designed for protection and performance in RF systems operating from DC up to 40 GHz.

WHITE PAPERS AND CASE STUDIES

NEWS

ABOUT

Harvest and Collection

Harvest and collection is the process used to retrieve biological agents and vaccines present in cell cultures or to harvest and collect stem cells from blood or bone marrow. Many bioresearch companies have sophisticated systems for harvesting and collecting cells. These systems make counting cells easier and they also protect against contamination.

Cells are harvested once the cells reach a density level in the cell culture medium that precludes further growth. The best time to harvest cells is when they are in a confluent state (at least 50% of the culture dish is covered and before 100% of the dish is covered).

Cells can be harvested using on of three methods:  Mechanical, using Proteolytic enzymes, or using EDTA.  Mechanical harvesting uses a rubber spatula to remove the cells from the growth surface or culture. This method is quick but can cause many cells to die because it is highly disruptive. This method is favored when harvesting lots of different samples of cells to prepare extracts.

In this case, viability of the cells doesn’t matter. Three enzymes; Trypsin, Collagenase, and Pronase can be used in combination with EDTA. The combination of these enzymes with the EDTA makes cells detach from the growth medium.

This method is easy but also has a downside. It can damage the cell surface by eating up exposed cell surface proteins. EDTA can be used alone to detach cells from their medium and it is gentler than using trypsin.

Normally, collected cells are placed in a new suspension, or growth medium to continue growing new cultures. This is referred to as passaging or splitting the cells.