Harvest & Collection

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    Compaction Simulation / Emulation

    Building on the capabilities of the NP-RD10A benchtop tablet press (discussed in our previous article on USP <1062>), our services are further enhanced by the integration of the Presster™ compaction emulator. This state-of-the-art tool is designed to replicate the compaction dynamics of production tablet presses in a laboratory setting. It allows for tangible fine-tuning of the compression cycle, providing an accurate depiction of how a tablting formulation will behave under commercial manufacturing conditions. Using the Presster, Natoli Scientific can simulate full-scale production, permitting us to forecast problems before they escalate to costly production delays. Our experts can optimize the tablet design for our clients, ensuring a smooth transition from laboratory to production while adhering to the rigorous standards set by the industry including the current USP <1062> chapter.

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    Pharmaceutical X-Ray Inspection System for Blister Packaging: XR75

    The XR75 Pharma X-ray is optimized for the quality control of pharmaceutical, nutraceutical, and cosmetic products in thin opaque packaging materials that cannot be inspected manually or with visual systems.

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    WR-15 (50-75GHz) Waveguide Twist: WT90-1500WG

    Marki Microwave waveguide twists provide accurate offsets and directional changes for waveguide transmission lines and are built with high precision and then gold plated for high corrosion resistance.

  • LAF Downflow Booths For Weighting and Sampling Powders
    LAF Downflow Booths For Weighing And Sampling Powders

    The LAF Downflow Booth provides product and personnel protection during the weighing or sampling of powdered substances through precise air circulation.

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    Oral Liquid Dosage Solutions Custom-Designed For Your Drug's Patients

    Oral non-sterile liquid manufacturing options offer a variety of liquid and suspension dosage solutions designed specifically around palatability — progressing from formulation development through full-scale commercial production.

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Harvest and Collection

Harvest and collection is the process used to retrieve biological agents and vaccines present in cell cultures or to harvest and collect stem cells from blood or bone marrow. Many bioresearch companies have sophisticated systems for harvesting and collecting cells. These systems make counting cells easier and they also protect against contamination.

Cells are harvested once the cells reach a density level in the cell culture medium that precludes further growth. The best time to harvest cells is when they are in a confluent state (at least 50% of the culture dish is covered and before 100% of the dish is covered).

Cells can be harvested using on of three methods:  Mechanical, using Proteolytic enzymes, or using EDTA.  Mechanical harvesting uses a rubber spatula to remove the cells from the growth surface or culture. This method is quick but can cause many cells to die because it is highly disruptive. This method is favored when harvesting lots of different samples of cells to prepare extracts.

In this case, viability of the cells doesn’t matter. Three enzymes; Trypsin, Collagenase, and Pronase can be used in combination with EDTA. The combination of these enzymes with the EDTA makes cells detach from the growth medium.

This method is easy but also has a downside. It can damage the cell surface by eating up exposed cell surface proteins. EDTA can be used alone to detach cells from their medium and it is gentler than using trypsin.

Normally, collected cells are placed in a new suspension, or growth medium to continue growing new cultures. This is referred to as passaging or splitting the cells.