Harvest & Collection

PRODUCTS

  • GettyImages-1025834378-manufacturing-excipient-drug-pharma
    Integrated DMPK Services For Lead Optimization, IND Filing

    Take advantage of our end-to-end DMPK expertise for optimizing compounds from discovery through IND filing for small and large molecules

  • Curebase app ePRO- feature
    A Single Digital Experience Participants Will Love

    Experience higher data quality, retention and participant compliance by providing a truly engaging experience from data submission to reminders, appointment management, telemedicine and more -- from the phone, tablet, or desktop.

  • GettyImages-669121480-pill-tablet-capsule-OSD-oral-solid
    Compaction Simulation / Emulation

    Building on the capabilities of the NP-RD10A benchtop tablet press (discussed in our previous article on USP <1062>), our services are further enhanced by the integration of the Presster™ compaction emulator. This state-of-the-art tool is designed to replicate the compaction dynamics of production tablet presses in a laboratory setting. It allows for tangible fine-tuning of the compression cycle, providing an accurate depiction of how a tablting formulation will behave under commercial manufacturing conditions. Using the Presster, Natoli Scientific can simulate full-scale production, permitting us to forecast problems before they escalate to costly production delays. Our experts can optimize the tablet design for our clients, ensuring a smooth transition from laboratory to production while adhering to the rigorous standards set by the industry including the current USP <1062> chapter.

  • GettyImages-2235582013-prefilled-syringe-liquid-manufacturing
    Oral Liquid Dosage Solutions Custom-Designed For Your Drug's Patients

    Oral non-sterile liquid manufacturing options offer a variety of liquid and suspension dosage solutions designed specifically around palatability — progressing from formulation development through full-scale commercial production.

  • S-Band GaN Solid State Power Amplifier (SSPA): VSS3607
    S-Band GaN Solid State 1.3 kW Power Amplifier Module: VSS3607

    CPI EDB presents the VSS3607 GaN solid state power amplifier (SSPAs) operating in the S-band (2.7 – 2.9 GHz). These high efficiency, high power, compact amplifiers are easy to maintain, and are designed for use in air surveillance radar applications.

WHITE PAPERS AND CASE STUDIES

NEWS

ABOUT

Harvest and Collection

Harvest and collection is the process used to retrieve biological agents and vaccines present in cell cultures or to harvest and collect stem cells from blood or bone marrow. Many bioresearch companies have sophisticated systems for harvesting and collecting cells. These systems make counting cells easier and they also protect against contamination.

Cells are harvested once the cells reach a density level in the cell culture medium that precludes further growth. The best time to harvest cells is when they are in a confluent state (at least 50% of the culture dish is covered and before 100% of the dish is covered).

Cells can be harvested using on of three methods:  Mechanical, using Proteolytic enzymes, or using EDTA.  Mechanical harvesting uses a rubber spatula to remove the cells from the growth surface or culture. This method is quick but can cause many cells to die because it is highly disruptive. This method is favored when harvesting lots of different samples of cells to prepare extracts.

In this case, viability of the cells doesn’t matter. Three enzymes; Trypsin, Collagenase, and Pronase can be used in combination with EDTA. The combination of these enzymes with the EDTA makes cells detach from the growth medium.

This method is easy but also has a downside. It can damage the cell surface by eating up exposed cell surface proteins. EDTA can be used alone to detach cells from their medium and it is gentler than using trypsin.

Normally, collected cells are placed in a new suspension, or growth medium to continue growing new cultures. This is referred to as passaging or splitting the cells.