Harvest & Collection

PRODUCTS

  • Flex-Line Feature
    Robotic Pharmaceutical Aseptic Filling System

    Fast-track your market entry with our robust aseptic filling platform. The Flex-Line™ Robotic Filler provides full integration of Scientific Products filling and Comecer isolation technologies for a seamless process from order through Validation.

  • GettyImages-2036497686-lab-team-collaboration-laboratory-tablet-computer
    Avance Clinical's Specialist Areas Of Focus

    Avance Clinical offers deep scientific expertise and extensive knowledge across a range of specialized therapeutic indications, consistently achieving global regulatory approvals.

  • Senior medical checkup patient and doctor-GettyImages-1938542779
    Ergomed's Patient-Centric Focus Drives Our Team

    Ergomed places patients and their care partners’ needs at the core of our services. We strive to build empathetic relationships with patients and their care partners, not just during the trial but also before and after. Our team provides personalized support, aiming to alleviate the burden of study participation. Ergomed and our trusted partners are deeply committed to bringing lifesaving treatments to patients.

  • Tulip Image 2
    Manufacture High-Quality Products With Streamlined Compliance

    Manufacture high-quality products with streamlined compliance.

  • GettyImages-1180581753-scientists-lab-monitoring-computer-laboratory
    Lab Equipment Monitoring For The Modern Lab

    Ensuring the integrity of results and maintaining regulatory compliance are paramount concerns for any lab today, especially with increasingly complex laboratory processes. Cloud-based device management offers several advantages for laboratory equipment monitoring.

WHITE PAPERS AND CASE STUDIES

NEWS

ABOUT

Harvest and Collection

Harvest and collection is the process used to retrieve biological agents and vaccines present in cell cultures or to harvest and collect stem cells from blood or bone marrow. Many bioresearch companies have sophisticated systems for harvesting and collecting cells. These systems make counting cells easier and they also protect against contamination.

Cells are harvested once the cells reach a density level in the cell culture medium that precludes further growth. The best time to harvest cells is when they are in a confluent state (at least 50% of the culture dish is covered and before 100% of the dish is covered).

Cells can be harvested using on of three methods:  Mechanical, using Proteolytic enzymes, or using EDTA.  Mechanical harvesting uses a rubber spatula to remove the cells from the growth surface or culture. This method is quick but can cause many cells to die because it is highly disruptive. This method is favored when harvesting lots of different samples of cells to prepare extracts.

In this case, viability of the cells doesn’t matter. Three enzymes; Trypsin, Collagenase, and Pronase can be used in combination with EDTA. The combination of these enzymes with the EDTA makes cells detach from the growth medium.

This method is easy but also has a downside. It can damage the cell surface by eating up exposed cell surface proteins. EDTA can be used alone to detach cells from their medium and it is gentler than using trypsin.

Normally, collected cells are placed in a new suspension, or growth medium to continue growing new cultures. This is referred to as passaging or splitting the cells.