Harvest & Collection

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    Building on the capabilities of the NP-RD10A benchtop tablet press (discussed in our previous article on USP <1062>), our services are further enhanced by the integration of the Presster™ compaction emulator. This state-of-the-art tool is designed to replicate the compaction dynamics of production tablet presses in a laboratory setting. It allows for tangible fine-tuning of the compression cycle, providing an accurate depiction of how a tablting formulation will behave under commercial manufacturing conditions. Using the Presster, Natoli Scientific can simulate full-scale production, permitting us to forecast problems before they escalate to costly production delays. Our experts can optimize the tablet design for our clients, ensuring a smooth transition from laboratory to production while adhering to the rigorous standards set by the industry including the current USP <1062> chapter.

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    The leading GIS-centric solution for permitting, licensing and land management—designed to simplify workflows for customers and staff. Improve public services and cross-team collaboration with a digital solution that keeps pace with the changes in your community.

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    The PBS-0.1 Mini single use bioreactor is compatible with 60mL-100mL working volumes and is the best starting point for optimizing cell therapies by leveraging the benefits of Vertical-Wheel mixing that can be easily scaled up for larger volumes.

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    PHCbi Humidified Plant Growth Chamber: MLR-352H-PA

    PHCbi brand's 10.4 cu.ft. (294 L) humidified plant growth chamber is designed to helps ensure highly accurate, reproducible chamber conditions. It allows for precise control of temperature, humidity, and lighting - making it ideal for drosophila breeding, diurnal growth studies, plant cell culturing and more. Robust cabinet construction with corrosion-resistant stainless-steel surfaces simplify cleaning and eliminate rust due to high humidity. It uses SNAP compliant, low flammability HFO refrigerants to assure low environmental impact.

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    1 - 22 GHz MMIC 2-Way High Isolation Power Divider/Power Splitter: MPBR-0122CSP3

    The MPBR-0122CSP3 is a compact, high-performance MMIC power divider/power splitter that operates across the 1 to 22 GHz frequency range.

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Harvest and Collection

Harvest and collection is the process used to retrieve biological agents and vaccines present in cell cultures or to harvest and collect stem cells from blood or bone marrow. Many bioresearch companies have sophisticated systems for harvesting and collecting cells. These systems make counting cells easier and they also protect against contamination.

Cells are harvested once the cells reach a density level in the cell culture medium that precludes further growth. The best time to harvest cells is when they are in a confluent state (at least 50% of the culture dish is covered and before 100% of the dish is covered).

Cells can be harvested using on of three methods:  Mechanical, using Proteolytic enzymes, or using EDTA.  Mechanical harvesting uses a rubber spatula to remove the cells from the growth surface or culture. This method is quick but can cause many cells to die because it is highly disruptive. This method is favored when harvesting lots of different samples of cells to prepare extracts.

In this case, viability of the cells doesn’t matter. Three enzymes; Trypsin, Collagenase, and Pronase can be used in combination with EDTA. The combination of these enzymes with the EDTA makes cells detach from the growth medium.

This method is easy but also has a downside. It can damage the cell surface by eating up exposed cell surface proteins. EDTA can be used alone to detach cells from their medium and it is gentler than using trypsin.

Normally, collected cells are placed in a new suspension, or growth medium to continue growing new cultures. This is referred to as passaging or splitting the cells.