Harvest & Collection

PRODUCTS

  • Office-meeting-GettyImages-1353983621
    Pre-Approval Inspection (PAI) Readiness

    Our team of experts are equipped to guide you through every step of any Inspection, especially Pre-Approval Inspections, due to the nature of their criticality.

  • GettyImages-2213157127-drug-formulation-discovery-pipette-liquid-vial
    Leaders In Oral Formulation

    Mikart’s development services include a range of pre-formulation capabilities for both oral solid and non-sterile oral liquid dose drug products.

  • Scientists working in lab-GettyImages-542935478
    From Design To Operation: Supporting GMP Facility Readiness

    For more than 20 years, Eurofins PSS Insourcing Solutions® has partnered with biotech and pharmaceutical companies to successfully validate and open new or remodeled facilities, meeting regulatory requirements. With a proven track record across numerous facility projects, our flexible, client-focused model ensures seamless integration with internal teams and third-party vendors, delivering high satisfaction and long-term partnerships.

  • GettyImages-1327977866-cell-culture-sample-laboratory
    Development Solutions For Biologics CHO CLD Platforms

    Aragen offers choice of CLD platforms, efficient Upstream and Downstream process development

  • SSV
    Pharmaceutical Checkweigher For Product Inspection

    The SSV-h Checkweigher is a great cost-effective solution for industrial, pharmaceutical, and packaged food products that need to meet stringent accuracy and reliability standards. It is equipped with a force balance load cell that produces faster response and readings with maximum accuracy of +/- 0.01 g. The Smart Measurement Function uses improved signal processing, filtering, and scale resolution to reduce rejections due to double product errors. The giveaway feature calculates product excess both as total weight and percentage, allowing you to adjust filling levels and reduce costs. The SSV-h series comes with an IP30 dustproof rating that makes it ideal for non-washdown applications. Its lightweight and rigid conveyor can be easily disassembled and wiped clean without any tools.

WHITE PAPERS AND CASE STUDIES

NEWS

ABOUT

Harvest and Collection

Harvest and collection is the process used to retrieve biological agents and vaccines present in cell cultures or to harvest and collect stem cells from blood or bone marrow. Many bioresearch companies have sophisticated systems for harvesting and collecting cells. These systems make counting cells easier and they also protect against contamination.

Cells are harvested once the cells reach a density level in the cell culture medium that precludes further growth. The best time to harvest cells is when they are in a confluent state (at least 50% of the culture dish is covered and before 100% of the dish is covered).

Cells can be harvested using on of three methods:  Mechanical, using Proteolytic enzymes, or using EDTA.  Mechanical harvesting uses a rubber spatula to remove the cells from the growth surface or culture. This method is quick but can cause many cells to die because it is highly disruptive. This method is favored when harvesting lots of different samples of cells to prepare extracts.

In this case, viability of the cells doesn’t matter. Three enzymes; Trypsin, Collagenase, and Pronase can be used in combination with EDTA. The combination of these enzymes with the EDTA makes cells detach from the growth medium.

This method is easy but also has a downside. It can damage the cell surface by eating up exposed cell surface proteins. EDTA can be used alone to detach cells from their medium and it is gentler than using trypsin.

Normally, collected cells are placed in a new suspension, or growth medium to continue growing new cultures. This is referred to as passaging or splitting the cells.