Harvest & Collection

PRODUCTS

  • Filamatic Filler, Model DAB-32-4
    Used Filamatic Aseptic Filling System: Model DAB-32-4

    Used Filamatic piston filler, model DAB-32-4, with (2) fill heads with pumps, on stand, 220 volt, serial# 022942.

  • X-Band Receiver Protector with Integral Noise Source: MA9905
    X-Band 2.5 kW Receiver Protector with Integral Noise Source: MA9905

    The MA9905 is an X-band receiver protector with an integral noise source. This receiver protector operates at 2.5 kW, and features a compact size for applications requiring less space.

  • Solid State Switches
    Solid State Switches

    CPI EDB offers solid state switches designed for operation in UFH-band, L-band, S-band, C-band, X-band, Ku-band, and Ka-band applications. With peak power handling ranging from 10 to 500 W, these switches are also capable of providing low loss with isolation up to 40 dB.

  • GettyImages-2155531148 electronic data, ePRO, eCOA
    eCOA / ePRO

    Empower participants, clinicians, and observers. Enable convenient and accurate outcome reporting, enhancing data quality and study efficiency with electronic patient reported outcomes (ePRO) and electronic clinical outcome assessments (eCOA).

  • amp20184
    0.8 - 2.5 GHz, 500 Watt Solid State High Power Amplifier: AMP20184

    The Exodus Advanced Communications AMP20184 is a solid-state, high-power amplifier designed for various applications, including EMI/RFI testing, laboratory use, CW/Pulse operations, and all communication needs.

WHITE PAPERS AND CASE STUDIES

NEWS

ABOUT

Harvest and Collection

Harvest and collection is the process used to retrieve biological agents and vaccines present in cell cultures or to harvest and collect stem cells from blood or bone marrow. Many bioresearch companies have sophisticated systems for harvesting and collecting cells. These systems make counting cells easier and they also protect against contamination.

Cells are harvested once the cells reach a density level in the cell culture medium that precludes further growth. The best time to harvest cells is when they are in a confluent state (at least 50% of the culture dish is covered and before 100% of the dish is covered).

Cells can be harvested using on of three methods:  Mechanical, using Proteolytic enzymes, or using EDTA.  Mechanical harvesting uses a rubber spatula to remove the cells from the growth surface or culture. This method is quick but can cause many cells to die because it is highly disruptive. This method is favored when harvesting lots of different samples of cells to prepare extracts.

In this case, viability of the cells doesn’t matter. Three enzymes; Trypsin, Collagenase, and Pronase can be used in combination with EDTA. The combination of these enzymes with the EDTA makes cells detach from the growth medium.

This method is easy but also has a downside. It can damage the cell surface by eating up exposed cell surface proteins. EDTA can be used alone to detach cells from their medium and it is gentler than using trypsin.

Normally, collected cells are placed in a new suspension, or growth medium to continue growing new cultures. This is referred to as passaging or splitting the cells.