Harvest & Collection

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  • MirusBio
    RevIT AAV Enhancer – 1.5 mL (For 1 L Of Cell Culture)

    Paired with any transfection reagent, the RevIT AAV Enhancer delivers higher titers for recombinant adeno-associated virus production.

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    Process Development

    EirGenix is a contract development and manufacturing organization that performs high quality and cost-effective services supporting our clients in development, analytical testing, and GMP manufacturing of biopharmaceuticals from pre-clinical to commercial manufacturing

    EirGenix has strong process development capabilities in both microbial and mammalian systems to provide our clients process development, process optimization, and process troubleshooting. We have successfully delivered results to meet clients’ requirement in product productivity, product quality, and process cost effectiveness. Our previous project experience includes recombinant proteins, plasmid DNA, fusion proteins, monoclonal antibodies, and biosimilars.

  • GettyImages-669121480-pill-tablet-capsule-OSD-oral-solid
    Compaction Simulation / Emulation

    Building on the capabilities of the NP-RD10A benchtop tablet press (discussed in our previous article on USP <1062>), our services are further enhanced by the integration of the Presster™ compaction emulator. This state-of-the-art tool is designed to replicate the compaction dynamics of production tablet presses in a laboratory setting. It allows for tangible fine-tuning of the compression cycle, providing an accurate depiction of how a tablting formulation will behave under commercial manufacturing conditions. Using the Presster, Natoli Scientific can simulate full-scale production, permitting us to forecast problems before they escalate to costly production delays. Our experts can optimize the tablet design for our clients, ensuring a smooth transition from laboratory to production while adhering to the rigorous standards set by the industry including the current USP <1062> chapter.

  • GettyImages-1087218908 In the Manufacturing Facility Team of Scientists Wearing Sterile Protective Coverall
    Biologics Manufacturing

    Recipharm Advanced Bio GMP manufacturing units focus on process robustness and consistency to ensure product quality, purity and efficacy. Our multi-product manufacturing facilities feature state-of-the-art equipment for animal cell-derived viral and microbial products for your clinical trials in phase 1-2 and in accordance with cGMP guidelines.

  • FMS Automation Module
    Pharmaceutical Automated Sample Handling System For Headspace Testing

    This collaborative automated sample handler eliminates manual sample handling enabling testing of large sample sets and collection of statistically significant data, while freeing analyst’s time. The FMS Automation Module has a sample capacity of hundreds of vials and can complete headspace testing with a throughput as high as 300 samples per hour.

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Harvest and Collection

Harvest and collection is the process used to retrieve biological agents and vaccines present in cell cultures or to harvest and collect stem cells from blood or bone marrow. Many bioresearch companies have sophisticated systems for harvesting and collecting cells. These systems make counting cells easier and they also protect against contamination.

Cells are harvested once the cells reach a density level in the cell culture medium that precludes further growth. The best time to harvest cells is when they are in a confluent state (at least 50% of the culture dish is covered and before 100% of the dish is covered).

Cells can be harvested using on of three methods:  Mechanical, using Proteolytic enzymes, or using EDTA.  Mechanical harvesting uses a rubber spatula to remove the cells from the growth surface or culture. This method is quick but can cause many cells to die because it is highly disruptive. This method is favored when harvesting lots of different samples of cells to prepare extracts.

In this case, viability of the cells doesn’t matter. Three enzymes; Trypsin, Collagenase, and Pronase can be used in combination with EDTA. The combination of these enzymes with the EDTA makes cells detach from the growth medium.

This method is easy but also has a downside. It can damage the cell surface by eating up exposed cell surface proteins. EDTA can be used alone to detach cells from their medium and it is gentler than using trypsin.

Normally, collected cells are placed in a new suspension, or growth medium to continue growing new cultures. This is referred to as passaging or splitting the cells.