Thermo Sci App Notes Bucket

  1. 2,000 L HyPerforma Single-Use Bioreactor Evaluation

    The stirred-tank reactor is the most commonly utilized reactor type in biotechnology. Read more about the Thermo Scientific™ HyPerforma™ Single-Use Bioreactor (S.U.B.). It is designed as a stirred-tank reactor for animal cell culture and has the unique advantage of utilizing completely single-use product contact surfaces.

  2. S.U.B. Enhancements For High-Density Perfusion Cultures

    This study presents how strategic enhancements to the sparge and agitation systems of Thermo Scientific HyPerforma S.U.B.s have revealed the potential for a three- to four-fold improvement in mixing and mass transfer performance compared to legacy S.U.B. designs.

  3. Mass Transfer Of Single-Use Fermenters To Stirred Glass Bioreactors

    The aggressive performance demands of industrial microbiology have limited the conversion of traditional fermentation processes into single-use systems. In order to address the unique needs of microbial applications, single-use fermentors have been designed to meet the requirements of microbial fermentation instead of being modified from a cell culture bioreactor.

  4. Scale-up And Tech Transfer From 100 L SIP/CIP Vessel To The 300 L S.U.F.

    A collaborative study with a new customer with the objective of taking an existing, smaller preclinical E. coli process previously performed in stainless steel vessels and scaling it up quickly using the Thermo Scientific™ HyPerforma™ 300 L Single-Use Fermentor (S.U.F.)

  5. Roller Bottles That Excel With ST, Vero, And MDBK Cells

    A study was conducted to compare the performance of Thermo Scientific Nunc Roller Bottles (1.2 and 2.5X) and 850 cm2 roller bottles from another supplier, for the culturing of ST, Vero, and MDBK cells.

  6. Rapid, Serum-Free, High-Yield Antibody Production In PETG Roller Bottles

    A rapid, flexible, and high-yield approach for both hybridoma expansion and for antibody production using a serum-free medium in Nunc PETG Roller Bottles.

  7. Nunc Cell Factory System Extractable Review

    The Nunc Cell Factory system minimizes the number of materials that could generate extractables by relying on ultrasonic welding to fuse individual layers of the Cell Factory system together.

  8. Master Cell Bank Production Of Liver Carcinoma Cells

    This application note reports on the initial experience of Thermo Scientific Nunc High Density Cell Factory systems, which enable large-scale production of adherent cells.

  9. Culture Of Human Mesenchymal Stem Cells In Nunc Cell Factory Systems

    Helpful methodology that can be applied to the large-scale expansion of other cell types for use in various applications using Cell Factory systems.

  10. Harvesting Adherent-Dependent Cells From Nunc Cell Factory Systems Protocol

    The Thermo Scientific™ Nunc™ Cell Factory™ system is a large multi-layer unit designed for the culture of adherent cells. The design of a Nunc Cell Factory system is similar to a cell culture flask, therefore, a standard cell harvesting protocol may be used. With minor modifications a standard protocol can be optimized for the unique features of the Nunc Cell Factory system.