Why Use The GSquad® Pro Vector System?

Expression vector design plays a critical role in the performance of a biomanufacturing campaign, influencing titer, product quality, and expression stability. In this video, Lonza's Peter O'Callaghan explores the latest evolution of the GS Gene Expression System® and the development of its upgraded GSPro® vector platform.

Drawing on decades of experience with the GS Gene Expression System®, Lonza's R&D teams developed LHP1, a high-strength gene promoter designed to enhance recombinant protein expression. O'Callaghan explains how LHP1 forms a key component of the GSPro® vector platform, which uses modular part vectors and destination vectors optimized for products containing one to four protein chains.

The video walks through the vector construction workflow, including standard cloning methods followed by one-pot Golden Gate assembly to create a GS piggyBac®-compatible expression vector. O'Callaghan also highlights Lonza's internal testing, which has demonstrated higher titers across a range of product types, along with improved product-quality attributes and strong expression stability compared with the previous promoter platform.

Watch the full video to learn how GSPro® and the LHP1 promoter advance expression performance and support biomanufacturing from development through commercial scale.