What Is Protein Nuclear Magnetic Resonance Spectroscopy?

Protein nuclear magnetic resonance spectroscopy, abbreviated protein NMR is a field of structural biology, that applies nuclear magnetic resonance spectroscopy to investigating proteins. The goal is to obtain information about the structure and dynamics of the proteins under investigation. The field was pioneered by, among others, Kurt Wüthrich, who won the Nobel prize in 2002, and is being continually used and improved in both academia and the biotech industry. Structure determination by nuclear magnetic resonance spectroscopy usually consists of several following phases, each using a separate set of highly specialized techniques. The sample is prepared, resonances are assigned, restraints are generated and a structure is calculated and validated.