Western Blot Handbook For Protein Detection And Quantification

This handbook provides a starting point to understand the principles behind this common technique for protein detection and quantification. Explore our step-by-step guide to execute a standard western blot protocol, complete with tips for troubleshooting. Learn how to accurately analyze data to achieve reliable and reproducible results. This handbook is suitable for both students and researchers new to western blotting and wishing to master the technique, and skilled scientists wanting a refresher.

Learn about:

• A brief history and the main principles behind western blotting
• ProteinSimple’s Simple Western platform (fully automated gel-free, blot-free westerns)
• Proper controls, antibody validation, and data normalization
• Traditional western blot workflow and standard protocol