Virus Reduction And Impurity Removal Potential Of Nuvia aPrime 4A Resin

This application note explores the virus reduction and impurity removal capabilities of Nuvia aPrime 4A Resin, a mixed-mode chromatography resin designed for downstream processing of monoclonal antibodies. Using a design of experiments (DOE) approach, the study evaluates how buffer pH and conductivity influence the clearance of minute virus of mice (MVM) and xenotropic murine leukemia virus (XMuLV), alongside host cell proteins, DNA, endotoxins, and aggregates. The resin’s dual hydrophobic and anion exchange properties enable selective binding and flow-through purification, with optimal virus clearance achieved at higher pH and salt concentrations.

For process developers seeking robust viral safety and impurity control, gain actionable insights into optimizing purification conditions.