VIPS Updates: Product Improvements For An Increased Efficiency In Single-Cell Seeding Process

VIPS (Verified In-Situ Plate Seeding) is the high efficiency single cell seeder designed to accelerate single-cell cloning workflows. The instrument launched in 2017 with the ambition of combining single-cell seeding with assurance of monoclonality and has become an integral part of major cell line development (CLD) workflows around the world.

VIPS uses a low seeding pressure of less than one psi in the dispensing of droplets to seed single cells. The resulting increase in high cell viability, and subsequent increase in clonal outgrowth, is an important foundation for high efficiency cell seeding workflows. Laboratories have confirmed that the increased seeding efficiency and percentage of wells confirmed with single cells, made possible with VIPS, results in a major transformational change to lab productivity. VIPS workflows regularly deliver ~80% seeding efficiency, accelerating workflows and reducing the number of plates required from the outset.

This paper highlights a number of updates to VIPS to improve the efficiency of single-cell seeding we have incorporated over the last year.