Anchorage-dependent cells, such as Vero cells, are widely used as a platform for viral vector production. To cultivate Vero cells in stirred-tank bioreactors, they require a growth matrix to attach to. The development of processes at bench scale is crucial to establish cost-efficient workflows in larger volumes. In this webinar, we demonstrate, how different process parameter can be monitored and controlled in a perfusion bioprocess to cultivate anchorage-dependent cells. We demonstrate the great potential of using packed-bed bioreactors or microcarrier spin filter for attachment cell-based vaccine production.
Key Learning Objectives: Importance of high-quality pre-cultures for bioreactor inoculation Importance of process monitoring and control Hands-on expertise on how to cultivate and grow anchorage dependent cells on different attachment matrixes.