Vero Cell-Based Vaccine Production: Cell lines, Media And Bioreactor Options

By Ma Sha

The Vero cell line is one of the most satisfactory vaccine production hosts based on its infectability, stability and well-documented performance in quality and quantity of viral yield. It is one of the first cell lines that received FDA approval for vaccine use and is used throughout the world. Cell culture media technology has advanced drastically in recent years, and a number of serum free and protein free options are available through commercial suppliers. Because serum tends to bind toxins and contaminants, its elimination calls for adoption of the highest quality reagents and careful monitoring of culture conditions in order to achieve optimal performance.

In this paper, we review the strategies for optimizing Vero cell based vaccine production using rabies and influenza as examples and highlight a combination of advances that provide strong support for the use of cell culture systems for virus vaccine production.