Utilizing Retrovirus-Like Particles (RVLP) To Evaluate Viral Clearance For Multiple Modes Of Separation
By David Cetlin, senior director, MockVĀ® products

Viral clearance studies are critical in ensuring the safety of biopharmaceutical products. Traditional methods often rely on infectious viruses, posing safety concerns and limitations. This poster introduces a novel approach using retrovirus-like particles (RVLPs) as a safe and effective alternative.
RVLP Production and Characterization
RVLPs are non-infectious particles that mimic the structure and behavior of retroviruses. They are produced in CHO cells and purified through various chromatographic methods. The poster presents data on RVLP production, purification, and characterization, including size and morphology analysis.
RVLPs in Viral Clearance Studies
The poster highlights the application of RVLPs in evaluating viral clearance for different separation modes, including:
- Viral Filtration
- Anion Exchange Chromatography
- Protein A Chromatography
- Hydrophobic Interaction Chromatography
- Cation Exchange Chromatography
- Mixed Mode Chromatography
Advantages of RVLPs
RVLPs offer several advantages over traditional methods, including:
- Safety: RVLPs are non-infectious, eliminating the risks associated with handling live viruses.
- Versatility: RVLPs can be used to evaluate various separation modes and process conditions.
- Sensitivity: RVLPs can be detected with high sensitivity using molecular methods.
- Relevance: RVLPs closely resemble retroviruses, providing relevant insights into viral clearance mechanisms.
RVLPs represent a promising tool for viral clearance studies, offering a safer, more versatile, and sensitive alternative to traditional methods. Their use can enhance the safety and efficiency of biopharmaceutical manufacturing processes.
Download the full poster to learn more about the production, characterization, and application of RVLPs in viral clearance evaluation.
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