Utilizing GORE® Protein Capture Device With Protein A For Rapid Process Development In Cell Line Optimization

By Jess Kettner, Jeffrey Cassel, and William C. Barrett, Ph.D, W. L. Gore & Associates, Inc

Accelerating cell line optimization is critical for biopharmaceutical development. Explore a high-throughput approach using the GORE® Protein Capture Device with Protein A. Designed for rapid purification of monoclonal antibodies, the membrane-based device enables processing of 24 samples—each 250 mL at 0.1 g/L titer—in under eight hours. The study demonstrates consistent yields of 95–97% across all cycles, highlighting the device’s efficiency and reliability. With short residence times and compatibility with standard FPLC systems, this solution eliminates the need for customized equipment while maintaining high productivity. Detailed cycling protocols and performance metrics underscore its value in early-stage process development. For teams focused on streamlining clone selection or cell line screening, gain actionable insights into reducing time-to-data without compromising quality.

Explore the full application note to see how rapid purification can transform your development timelines.