Using Macro Mass Photometry To Swiftly Distinguish And Quantify Particles In Adenoviral (AdV) vectors Capsids

By Julia Talarek and Shawkat Hussain, Jenner Institute, University of Oxford, UK, and Ivor Crooks, Matthew Powell-Grabaskey and Matt Ranaghan, Refeyn Ltd., UK

This study explores the application of macro mass photometry, specifically via the Karitro^MP benchtop instrument, in analyzing Adenoviral (AdV) vectors - essential components in vaccines and gene therapy. This technology can differentiate and quantify particles in AdV samples, distinguishing between empty and full viral capsids, and accurately measuring the proportion of full capsids present. The Karitro^MP's results align with other orthogonal techniques such as analytical ultracentrifugation and negative stain electron microscopy. Macro mass photometry can also discern between full AdV containing a target gene and helper AdV. The study concludes that the Karitro^MP macro mass photometer is a vital tool for AdV analysis, streamlining R&D, and process development to optimize adenovirus production through quick and accurate sample purity assessment.