Use Of The Agilent 4200 TapeStation System For Sample Quality Control In The Whole-Genome Sequencing Workflow At The German Cancer Research Center (DKFZ)

Reliable sample quality control is essential for successful next‑generation sequencing, especially when working with challenging FFPE‑derived DNA. This application note demonstrates how comprehensive QC at every stage of an exome sequencing workflow—starting from incoming gDNA assessment through final library evaluation—supports consistent, high‑confidence sequencing outcomes. It highlights how integrity scoring, precise sizing, and accurate quantification guide decisions on fragmentation protocols, enrichment steps, and library pooling. Even samples with low integrity can yield meaningful sequencing data when monitored with robust QC checkpoints. Walk through real sample data, troubleshooting insights, and criteria for evaluating libraries and sequencing performance.

Take a closer look at examples and QC thresholds used across the workflow.