UPLC-MS/MS Determination Of GLP-1 Analogue, Liraglutide A Bioactive Peptide In Human Plasma

By Taposh Gorella, P.M.N. Rajesh, and Padmakar Wagh, Waters Corporation

This application note showcases a streamlined and effective approach for the sensitive and reproducible analysis of liraglutide in human plasma. It integrates a straightforward sample pretreatment method with solid-phase extraction (SPE) and analytical flow liquid chromatography (LC) coupled with tandem quadrupole mass spectrometry (MS). The process begins with protein precipitation, which is then followed by a mixed-mode SPE conducted in the μElution format. This combination eliminates the need for sample evaporation—a step that often leads to analyte loss due to adsorption—thereby enhancing the overall recovery and reliability of the assay.

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