Unlocking The Potential Of Electroporation For Studying Ion Channel-Related Disorders And iPSCs

Genetic disorders such as cystic fibrosis and long QT syndrome often result from mutations in ion channel and transporter genes. Electroporation, a non-viral technique, is increasingly used to study these conditions. By applying electric pulses, cells temporarily become permeable, allowing the introduction of genetic material. This method supports the creation of stable cell lines with specific traits or the direct delivery of therapeutic genes to target cells.

Despite its potential, electroporation success can vary based on cell type and viability, and scaling the process for clinical use poses challenges.

In this webinar, Dr. Alfred George presents an innovative electroporation approach addressing these obstacles. He highlights MaxCyte electroporation technology, which enables the expression of human ion channel variants in mammalian cells. This technique streamlines high-throughput automated patch clamp recording, allowing functional analysis of ion channel variants without requiring stable cell line development.

Discover how electroporation can introduce or correct mutations in induced pluripotent stem cells (iPSCs), providing a versatile tool for modeling genetic diseases and advancing gene therapy proof-of-concept studies.