Tumor - Apoptosis Detection Kits

TumorTACS is a complete reagent kit providing rapid and convenient identification of apop-tosis in tumors or cancer cells. The labeling of tumor-containing specimens can be prob-lematic due to the high levels of necrosis in these tissues. During necrosis, cessation of homeostasis, cell swelling, and nuclear membrane rupture generates highly degraded DNA that can interfere with interpretation of data. Trevigen's kit has a unique TUNEL-based sys-tem that preferentially labels the double-stranded DNA found in apoptotic cells. DNA frag-ments generated during apoptosis are end-labeled with modified nucleotides using a highly purified terminal deoxynucleotidyl transferase enzyme (TdT) in a unique non-toxic labeling buffer supplemented with cations for apoptosis-specific labeling. The incorporated nucleotides are subsequently detected using a horseradish peroxidase conjugate. The con-jugate catalyzes the conversion of diaminobenzidine (DAB) into a visual dark brown precip-itate. The kit includes methyl green counterstain which is a general counterstain appropri-ate for a wide variety of tissues and tumors types and provides good contrast with the brown DAB in labeled cells. TumorTACS contains all the reagents required for permeabiliz-ing, labeling, staining and counterstaining tumor samples, including TACS-Nuclease for preparing positive controls.

Features:

• Fast. Requires less than 3 hours to complete.
• Exclusive, non-toxic TACS Safe TdT buffer - sodium cacodylate free.
• Unique buffer system produces more consistent labeling.
• Performance tested on tumor samples.
• Includes exclusive Cytonin permeabilization reagent.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.

Applications:

• In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
• Assists in the identification of apoptotic morphologies.
• Helps resolve unique problems encountered when using tissues or cells from tumors.

Components:

• Permeabilization reagents
• Labeling enzyme
• Chromogenic substrate
• Labeling buffer
• Optimized cation
• Counterstain
• Nucleotide mix
• Detection reagents
• TACS-Nuclease

Items not included: Reagents for dehydration and rehydration, H²O², PBS buffer, and disposables.

Related Products:

• Cell Proliferation Assays
• DNA Laddering kits
• Services and Assessories

Storage: Components are stored at -20°C, 4°C and room temperature.

N/A, 8405 Helgerman Ct., Gaithersburg, MD 20877. Tel: 800-873-8443; Fax: 301-216-2801.