Top 5 Things to Know for Successful Pichia Fermentation

By Tony Allman

How can your organization optimize the fermentation of pichia pastoris through advances hardware capabilities?

The cultivation of Pichia in a bioreactor comes with few challenges including a need for high rates of oxygen transfer and different feeding strategies, the generation of surplus heat, and foam formation. Looking deeper into the individual challenges helps researchers to find solutions using bioprocess equipment. Some of the key factors of importance that have been identified include:

• The need for a powerful drive motor and optimized impellor system
• The importance of efficient cooling
• A need for high accuracy pumps which can be re-allocated for Pichia fermentation “out of the box”
• The importance of both local and supervisory control software
• The value of additional sensors to detect cell growth rate and/or metabolic processes