By Peter A. Fung, PhD
First published November 9, 2015 on www.Biocompare.com
Liquid chromatography is a widely used and trusted methodology for sample separation that researchers utilize during a target-purification workflow. The basis for separation involves the interaction of a mobile sample (in solution) with a stationary or solid phase that will selectively capture, bind or interact with components in the sample. Chromatography takes advantage of the diverse chemical and physical properties of nucleic acids, small and large molecules and proteins to selectively exclude or capture targets of interest from sample mixtures.