Article | January 21, 2017

Tips For Preparing And Using Buffers With Your Chromatography System

Source: Bio-Rad Laboratories, Inc.

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By Terri Thompson
First published March 14, 2016 on www.Biocompare.com

Chromatography is a popular purification technique used in labs all over the world. If the system is setup and operated correctly, it can isolate desired compounds from a mixture in no time at all. Although, like many methods of purification, the quick and efficient setup and maintenance of your system is something that can a bit of time to master. With that said, one of the important and often overlooked tips you can do to increase the performance of your system is understand how to use and prepare your buffers and solvents. Even the simplest element such as buffer preparation, if not done well, could cause inconsistent results and loss of precious sample. Accurate preparation and correct selection of buffers is essential to obtain reproducible results in chromatography. Here we present some fundamental tips and tricks of buffer preparation to help you get the best separation of your compounds.