The Impact Of Elution pH On Product Quality Of Fc-Containing Proteins

By Alvaro Cruz-Izquierdo, Johanna Howson, Benjamin Summers, Jade Hopkins, Mark Hicks, and Hans Johansson

There is a large diversity in the Fc-containing molecules, but also in the cell culture feeds that produce them. This diversity is not just a challenge, but also an opportunity for the resin manufacturing industry to innovate and evolve. Protein A affinity chromatography is the most widely employed technique to purify Fc-containing proteins and antibodies due to its high specificity. While the elution of the target molecule is triggered by lowering the pH up to 3-3.5, this does not always create the optimum conditions for the target molecule and to remove impurities (aggregates, host cell proteins (HCP), and DNA).

Discover the next generation of affinity resins—DurA Cycle A50— and download the latest scientific poster from Purolite here. The poster contains an introduction to our latest product, DurA Cycle, and also explains the benefits of achieving acid-labile molecules and overall impurities using a milder elution pH with Praesto™ Jetted A50 HipH.

What is DurA Cycle A50?

DurA Cycle A50 is the latest generation protein A resin, designed for the purification of a wide range of mAbs and related constructs. DurA Cycle A50 has been specifically designed to address the need for increased stability to protease cleavage in challenging harvests and for the use of higher concentrations of sodium hydroxide, addressing the challenge of more complex purifications. Find out more.