Effects Of Conductivity On Dynamic Binding Capacity On CEX Resins

By Bio-Rad Laboratories, Inc

In an effort to improve the antibody manufacturing platform, newer Cation Exchange Resins were evaluated. Dynamic binding studies were conducted using 2 different monoclonal antibodies at a stable pH and varying conductivities across nine resins to determine the differences in binding capacity. DBC Studies were performed at a pH of 5 and conductivities of 5, 10 and 15 mS/cm for the nine cation exchange resins with breakthrough criteria set at 10%. While most of the resins provided excellent binding capacity (>50 g/L), the impact of conductivity on the dynamic binding capacity was shown to be very molecule dependent and varied significantly with the resin type.