The DAWN-ing Of A New Era - Wyatt Makes Absolute Particle Sizing Easy
Individually, both instruments offer state-of-the-art functionality, but when combined, they form the ultimate package for the biotechnology and pharmaceutical applications. By integrating the Eclipse Field Flow Fractionation with the DAWN Multi-Angle Light Scattering detection, reproducible absolute particle sizing can be achieved without the need for calibration or sizing standards.
The DAWN EOS is the world's most advanced commercial instrument for absolute macromolecular characterization. It is the only multi-angle light scattering (MALS) detector capable of determining radius and molar mass results simultaneously, and is able to calculate masses directly from the variation of the light scattering pattern. It works with absolute precision, meaning that there is no need to refer to standards or assumptions a priori.
Due to its versatility, the Eclipse particle fractionation device, plays a significant role in a wide range of research and development, including molecular biology, nanotechnology and environmental analysis. It produces extraordinary—yet extraordinarily gentle—particle and molecular separation for samples in the sub-micrometer range via a Field-Flow-Fractionation (FFF) channel. Moreover, as the process is rapid and non-destructive, the sample remains unaltered by a stationary phase.
The Eclipse offers distinct advantages over conventional particle separation apparatus, like Hydrodynamic Chromotography (HDC) instruments. Only the Eclipse plus the DAWN can obtain particle sizes without the need for channel calibration with arbitrary standards. Wyatt Technology has also incorporated various safety features into the design of the Eclipse, such as automatic shut-down and a pressure relief-valve actuation. In addition, the instruments are easy to service and incredibly robust. The Eclipse takes up little bench space and can be stacked with the DAWN EOS and other Wyatt detectors.
Taking such features into account, it was natural for Wyatt Technology to combine the separating powers of FFF with the measuring powers of MALS, to establish the ultimate separation system. By connecting the DAWN to the Eclipse, its resolution is dramatically increased to equal that of ultracentrifugation. Yet with their collective speed, flexibility, and ease of use, the DAWN and the Eclipse surpass even this benchmark.
Following twenty years of research and development, Wyatt Technology has designed the only integrated turn-key approach available, resulting in a system that can solve even the most difficult sizing tasks in the macromolecular and colloidal arena. The Eclipse and the DAWN EOS have been designed for maximum simplicity and automation, ensuring their place in any sophisticated research laboratory.