The Complete Guide To Western Blot

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Western blotting is a powerful analytical technique used to detect specific proteins within a complex mixture. It involves separating proteins by size through gel electrophoresis, followed by transferring them onto a membrane and probing with highly specific antibodies that bind to the target protein. This method not only confirms the presence of a protein but also provides insights into its relative abundance and molecular weight.

Whether you're validating experimental results or investigating protein expression, mastering Western blotting is essential for accurate and reliable data in molecular biology.