Technical Note: Nuvia™ S Media — A High-Capacity Cation Exchanger For Process Purification Of Monoclonal Antibodies

Nuvia S is a high-capacity strong cation exchanger designed for optimal bioprocessing performance. It is built on the macroporous rigid UNOsphere™ base matrix, through the incorporation of polymeric surface extenders with optimized density of sulfonate groups.

This application note describes the effects of flow rate, load pH, and conductivity on the binding of polyclonal human immunoglobulin G (hulgG) and monoclonal antibodies (mAbs) by this new chromatographic media. Preliminary studies on the removal of product-related impurities using Nuvia S were also conducted.