Taking A Hybrid Approach For Optimizing Transient Protein Expression In CHO Cells

When choosing between protein expression formats such as transient versus stable pools, considerations include speed, titre, and product quality. In this presentation, we show how we have optimized the transient expression workflow for the GS Xceed^® CHOK1SV GS-KO^® cell line by exploring the design space between transient protein expression and the construction of stable pools. We present GS Discovery^® which offers a ‘hybrid’ approach for boosting titres of a wide range of molecules.