Successfully Cultivate Hybridoma Cells In High Densities

By Ray Rose, Stacey Willard, and Ma Sha

The Fibra-Cel packed-bed basket technology has been established as an excellent method for the growth of suspension and anchorage-dependent cell lines. The three dimensional structure of the Fibra-Cel disk provides an excellent solid-support matrix for the entrapment or attachment of animal cells, allowing constant perfusion of nutrients in a low-shear environment. In this application note, we show that Hybridoma cells can be successfully cultivated in high densities in the 2.5 L packed-bed FibraCel basket controlled by a CelliGen 310 bioreactor.