Substantially Improve Protein A Elution: Fight HCP & DNA Contamination

Today, turbidity after viral inactivation, elevated HCP and DNA contamination of the product downstream of Protein A elution are serious problems plaguing mAb production processes that require costly and complex polishing trains. 

Here we investigate a novel approach that significantly increases the performance of the mAb purification process by dramatically enhancing the efficiency of the Protein A column and producing a substantially pharmaceutically pure product immediately after Protein A elution.

While the exact causes and mechanisms of the decrease in Protein A column performance are still a very active topic of research, high concentrations of chromatin in cell culture fluid has been suggested to be a major agent that interferes with Protein A chromatography, and acts as a precipitation catalyst at various stages of the process (1,2).
To decrease the chromatin content of the cell culture fluid...