Streamlining Discovery And Development For ncAA Incorporation

Choosing the right microbial expression host shapes every downstream decision in your protein production workflow, from early strain development through process scale-up and GMP manufacturing. Pseudomonas fluorescens is not a repurposed laboratory workhorse; it was identified and selected precisely because of its capacity for high-level protein expression, then systematically engineered to maximize that capability.

This presentation outlines how a combinatorial strain engineering approach, informed by multiomics data, has been applied to push expression performance well beyond what conventional hosts typically achieve. The workflow spans strain design through process development, analytical development, and into GMP-enabled manufacturing — a continuous development arc that reduces the handoff risks common in fragmented development models.

For biologics developers evaluating expression platforms early in their programs, the choice of host organism carries downstream consequences for yield, scalability, and manufacturing readiness. A purpose-built system designed from the organism up offers a different risk profile than one adapted from an existing platform.

To learn more, please access the full presentation below.