Sodium Hydroxide (NaOH) Exposure Conditions For Successful Operation Of Protein Capture Devices

By Jared Clinger, B.S., Jessica Kettner and William C. Barrett, Ph.D., W.L. Gore & Associates, Inc.

This application note provides data to support sodium hydroxide (NaOH) exposure limits for the 1.0mL and 3.5mL GORE Protein Capture Devices. The cycling protocol utilizing 0.52 molar (M) NaOH supports 50 purification cycles with minimal impact to purification yield, device capacity, or assessed purity. The method provided demonstrates that up to 50 purification cycles can be performed with a three minute 0.5M NaOH contract time per cycle.