Application Note

Simple Western Unravels The Signaling Web In Stem Cells For Regenerative Medicine

Source: bio-techne
Regenerative medicine and therapeutic stem cell therapy GettyImages-1334489810

The reprogramming of human-induced pluripotent stem cells (iPSCs) has revolutionized regenerative medicine, but gaining an accurate and quantitative understanding of signaling networks that regulate stem cell differentiation is crucial and remains a daunting challenge. The complexity of signaling networks stems from the many proteins and phosphorylated protein isoforms involved, and researchers are often challenged with limited material for analysis. Traditional methods like Western blot lack throughput, multiplexing ability, detection sensitivity, and reproducible quantification needed to gain a complete understanding of stem cell biology.

We show how Simple Western has unrivaled multiplex flexibility, sensitivity, and reproducibility for accurate protein expression measurements that scale with manufacturing workflows for stem cell therapy and regenerative medicine.

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