Application Note: Characterization Of Microbial Growth Profiles

On-line detection of oxygen and pH during culture in shake flasks

Shake flasks are the most commonly used cultivation systems for process and media optimization, screening for new products and strains as well as for the supply of inoculum for bioreactor cultivation. Oxygen limitation is one of the major though often neglected problems in aerobic shake flask cultivation. The new technology of the Shake Flask Reader SFR is an ideal tool for non-invasive monitoring of the two culture parameters oxygen and pH, and in addition reveals a detailed picture of growth performance and profiles.