Sensitive And Robust CAR Expression Detection Using Site-Specific Fluorescent Labeled Proteins And Flow Cytometry

By Siqi Huang, Xiaojuan Shi, Yanke Liu, Jane Liu, Spencer Chiang, ACROBiosystems

Accurate CAR expression detection is vital for ensuring the safety and efficacy of CAR cell therapies. ​While flow cytometry is widely used for this purpose, traditional fluorescent labeling methods often introduce variability, impacting assay reliability. ​Site-specific fluorescent labeling offers a transformative solution by preserving protein integrity, minimizing non-specific binding, and delivering consistent results across batches.

This approach enhances sensitivity and specificity, making it ideal for robust CAR expression detection in manufacturing settings. ​By addressing common challenges such as background noise and variability, site-specific labeling ensures precise evaluations critical for cell therapy development. ​

Discover how site-specific fluorescent labeling can revolutionize CAR expression detection and support the advancement of cell therapy manufacturing. ​ Access the full content for detailed insights and practical applications.