Scaling-Up Of The Bioluminescent Strain Aliivibrio sp. (ref. 1485) From Blue Amazon: Implications For The Formulation Of Sustainable Signaling Devices

By Jéssica Scherer, Mário Luiz Conte da Frota Júnior, Andressa de Moura Silva, Dayse Pereira Dias Silva, Laura Fernanda Costantin, Alexandre José Macedo, and Vanessa Ochi Agostini.

Developing sustainable signaling systems requires robust and scalable bioprocesses for novel biomaterials, such as bioluminescent marine bacteria. This application note validates an efficient, reproducible scaling-up protocol for the high light-emitting strain, Aliivibrio sp. (ref. 1485), using the INFORS HT Minifors 2 bioreactor under controlled operational conditions (28°C and pH 6.50).

The results show successful biomass production, reaching the stationary phase at approximately 68 hours (OD_600nm ≈1.2). However, the light kinetics demonstrated an inverse proportionality to cell growth. The maximum luminescence (276,000 photons) was reached rapidly at 3 hours, suggesting a divergence from the canonical Quorum Sensing control and a "switching off" effect due to metabolic repression at high cell density. This finding establishes the ideal harvesting window as immediate (0–5 hours) to capture peak performance in the commercial medium. For the necessary long-term stability in the final application, REGENERA is strategically transitioning to its proprietary, optimized medium (BO1485) designed to maintain light viability for up to 100 days.

Access the full application note to gain detailed insights into optimizing bioprocesses for next-generation biological lighting technologies.