Scalable Expansion Of Human Pluripotent Stem Cells

By Ruth Olmer, et al

The routine application of human pluripotent stem cells and their derivatives in regenerative medicine and innovative drug discovery will require the constant supply of high cell numbers in consistent, high quality. Well monitored and controlled stirredtank bioreactors represent suitable systems to establish upscalable bioprocesses enabling the required cell production. The following application note describes the successful cultivation of human pluripotent stem cells in suspension culture using Eppendorf BioBLU 0.3 Singleuse Vessels in a DASbox® Parallel Mini Bioreactor System.