Scalable Electroporation Optimizes Transposon Delivery In CAR T Cell Manufacturing Workflows

Transposon systems have become a proven and efficient method of gene transfer in the development of autologous cell therapies. These systems are more cost-effective and present fewer manufacturing complexities when compared to viral vectors.

This video details a study that optimized the delivery of the piggyBac transposon system for engineering cell lines and primary T cells. Learn how researchers used electroporation to achieve efficient delivery of the transposon, and see how they optimized a variety of conditions to balance transposition efficiency, durability of expression, and cell expansion. You will also learn how this method can support consistent transgene expression and cell viability across different donors.

Watch below to understand how optimizing electroporation parameters can lead to more consistent and robust CAR T cell manufacturing workflows.