SAFER Detection™ For Efficient Interrogation Of DNA Rearrangements In Gene-Edited Human Cells

CRISPR/Cas9-mediated gene editing has shown tremendous promise in treating genetic diseases. However, off-target editing and the potential for chromosomal rearrangements remain a safety concern. In this study, we introduce SAFER Detection™ (Selective Amplification for Efficient Rearrangement Detection), a tagmentation and next-generation sequencing-based method designed to enable quantitative detection of chromosomal rearrangement breakpoints with single base resolution.

SAFER Detection is capable of classifying rearrangements resulting from on-target and off-target editing by programmable nucleases such as CRISPR/Cas nucleases and TALENs. SAFER Detection exhibits high sensitivity in detecting intra-chromosomal and inter-chromosomal rearrangements mediated by off-target activity and DNA-damage repair pathways and is applicable to samples containing low cell numbers. When combined with a sensitive off-target nomination technology such as ONE-seq™, SAFER Detection provides a valuable method to assess the risk of chromosomal rearrangements in therapeutic genome editing.

Download the poster to learn more about SAFER Detection and its applications in gene editing.