RNAspectra Fluorescent Differential Display Kit
The RNAspectra Kits feature a new generation of Differential Display technology based on fluorescent detection of PCR products instead of radioactivity. It is the first non-radioactive differential display system with similiar sensitivity to that of 33P isotopic labeling.
Each kit contains all the reagents needed for fluorescent differential display except Taq DNA polymerase and a fluorescent scanner. The MessageClean® is highly recommended prior to differential display to remove trace amounts of DNA contamination from RNA samples.
As in the RNAimage® Kit, the RNAspectra kit contains three one-base-anchored oligo-dT primers to subdivide the mRNA population, except they are labeled with 5' Fluorescein. The use of three one-base-anchored primers reduces the redundancy and potential under-representation of the sub-population of mRNAs encountered by using either twelve individual or four degenerate two-base-anchored oligo-dT primers. With built-in restriction sites at the 5' ends of both anchored and arbitrary 13mers, the longer primer pairs produce highly selective and reproducible cDNA patterns. These improvements ensure that differentially expressed genes are more readily identified, cloned, and manipulated.
See Technical Notes Section of our website for further discussion on primer design, calculation of coverage, comparison of DD to other methodologies, and false positives.
GenHunter Corp., 624 Grassmere Park Drive, Suite 17, Nashville, TN 37211. Tel: 615-833-0665; Fax: 615-832-9461.