Revert™ Total Protein Stain Normalization Protocol

Accurate protein quantification in Western blotting hinges on reliable normalization, and total protein staining has emerged as the preferred method for correcting sample-to-sample and lane-to-lane variation. This manual provides detailed guidance for using Revert™ 700 and 520 Total Protein Stains to achieve consistent, reproducible results. It outlines pre- and post-immunodetection workflows, imaging techniques, and data analysis strategies—including lane normalization factor calculations and %CV assessments—to ensure meaningful interpretation of protein expression changes. Emphasis is placed on maintaining linear detection ranges, performing technical replicates, and validating uniform sample loading.

Access the full manual to learn how to strengthen your normalization strategy and data confidence.