Reference Methods For Packing Columns With Nuvia IMAC Resin

Immobilized metal affinity chromatography (IMAC) is an effective chromatography tool for preparing recombinant proteins naturally rich in histidine/cysteine residues, as well as those with histidine residue cluster engineered to their N- or C-terminus via recombinant DNA technology. While a small quantity of protein prepared on a laboratory bench is often sufficient for structural and functional characterization studies, process-scale columns are needed in the manufacturing of recombinant proteins for therapeutic applications. We have developed effective protocols for packing Nuvia IMAC Resin in various column sized to fulfill the needs of lab-scale to commercial production.