Recombinant Caspase Kits

BIOMOL offers three complete drug discovery kits for assaying the activity of Caspase-1, 3 and 8. These three QuantiZyme™ Assay Kits for Drug Discovery are useful in screening for inhibitors of Caspase-1, 3 or 8, important therapeutic targets involved in apoptotic pathways.

Caspase-1 (Interleukin-1Beta converting enzyme; ICE), the prototypical member of the ICE family of cysteine proteases, is the novel protease cleaves pro-interleukin-1Beta after Asp-116.

Caspase-3 (CPP32/Yama/Apopain) is one of the principal caspase activities found in apoptic cells and cleaves targets such as poly (ADP-ribose) polymerase (PARP) and nuclear lamins.

Caspase-8 (FLICE/MACH/Mch5) is implicated as the apical signaling protease in Fas- and TNF-receptor mediated apoptis and may activate downstream caspase zymogens such as procapase-3.

Kits include the following components:

• purified caspase-3 or -8 enzyme (human, recombinant)
• colormetric substrate (Ac DEVD-pNA, Ac-IETD-pNA, or Ac-YVAD-pNA)
• calibration standard (p-nitroaniline)
• inhibitor (aldehyde)
• assay buffer
• 1/2 volume 96-well microtiter plate
• instruction booklet

Each kit includes active caspase enzyme, caspase-specific substrate, calibration standard, aldehyde inhibitor, assay butter, 1/2 volume microtiter plate and instruction booklet: everything needed to study caspase 1, 3 or 8 activity.

BIOMOL Research Laboratories, Inc., 5120 Butler Pike, Plymouth Meeting, PA 19462. Tel: 800-9420430; Fax: 610-941-9252.