Whey proteins play a vital role in the formulation of food supplements because of numerous desirable nutritional and functional properties. The objective of this study was to develop a separation process to fractionate usable proteins from whey. First, total whey protein isolate (WPI) was purified because of its high-end functional advantages, as evidenced by a demand from nutrition and health segments. Second, ALA and BLG were purified because of their value in infant formula and confections, respectively.