Rapid Cycling Using A Protein Capture Device With Protein A, 58 mL For Clinical Applications

By Mike McManaway, Brad Joines and William C. Barrett, Ph.D., W. L. Gore & Associates, Inc.

Similar to the smaller scale GORE Protein Capture Devices that can be operated at faster flow rates (see Application Note PB8433, Increasing the Productivity of mAb Purification using the GORE Protein Capture Devices with Protein A), this evaluation demonstrates the capability to perform non-loading steps at flow rates up to 348 mL/min equivalent to 10 seconds residence time.

Here, we review if a shorter residence time could be used for more rapid cycling without impacting elution width, yield, Protein A leaching or HCP removal. In this cycling study, all but the loading steps were run at 10 srt and fractions analyzed for yield, elution width, Protein A leaching, and HCP LRV. The data indicates that the increased speed did not impact product quality or yield.